TABLE 2.

pH-dependent oligomerization of PvS1_FL and PvS1_ΔBelt^a

Protein	Theor. MW (monomer)	pH 5	pH 8
PvS1FL	67.71 kDa	135.8 ± 2.8 kDa	67.9 ± 1.1 kDa
PvS1ΔBelt	56.57 kDa	57.5 ± 0.2 kDa	57.5 ± 4.5 kDa

^a Molecular weight determination of PvS1_FL and PvS1_ΔBelt variants by analytical ultracentrifugation sedimentation equilibrium at pH 5 and pH 8, corresponding to the AUC profiles shown in Fig. 3. The theoretical molecular weights were determined from the amino acid sequence of the proteins. Error estimates were calculated using a Monte-Carlo simulation of 10,000 replicates.