Fig 4.
UppSUN increases membrane permeability in ΔmlaF mutants. (A and B) A. baumannii ATCC 17978VU (A) and 17978UN (B) wild-type and ΔmlaF strains with the endogenous or alternate uppS allele were incubated with efflux pump inhibitor CCCP and ethidium bromide and fluorescence was measured. (C and D) Quantified area under the curve for A and B, respectively. Significance is by one-way ANOVA with Tukey’s multiple comparisons test. All strains were significantly higher than the PBS control (P < 0.001). Comparisons between ΔmlaF strains with their respective wild-type strain are shown. Significant differences are indicated by *P < 0.05, ***P < 0.001, ****P < 0.0001. Data are means ± SEM, n = 3. Experiments were conducted two independent times with similar results.