Fig 5.
Isoprenoid biosynthesis suppressor mutations only confer increased resistance to SDS/EDTA in A. baumannii ATCC 17978 ΔmlaF strains with UppSUN. (A) Schematic of the isoprenoid biosynthetic pathway with key genes highlighted in red. (B and C) Wild-type and mutant strains were serially diluted before spotting on LB plates without (B) and with (C) 0.01% SDS and 0.15 mM EDTA. Experiments were conducted four independent times (n = 8) with similar results. (D–G) 17978VU (D and E) and 17978UN (F and G) wild-type and mutant strains were grown in LB without (D and F) or with (E and G) 0.01% SDS and 0.175 mM EDTA. Data are means ± SEM, n = 3.