Colonization mutants affect B. cereus colony expansion. Colony expansion of B. cereus in 1/10th-strength tryptic soy agar (TSA) was observed in the presence of wild-type and mutant F. johnsoniae strains. The area of the B. cereus colony is shown as a box plot (A), the experiment was performed three times, and a representative image is shown in panel B. The values (cm2) were square-root transformed for equal variance, and the statistical significance was evaluated with a one-way ANOVA followed by Dunnett’s test. Differences between the mutants and wild type and between the wild type and B. cereus alone are indicated as ns, not significant; *, P
< 0.05; **, P
< 0.01, or ****, P
< 0.0001.