TABLE 1.

Sand colonization genes identified using INSeq screen and validated by mutant analysis^a

Accession ID	Gene	Annotation	COG category	Log2 fold change (input/output)
Fjoh_0334	FJOH_RS01770	DegT/DnrJ/EryC1/StrS family aminotransferase	E	2.54
Fjoh_0651	FJOH_RS03415	Hypothetical protein	No COG	2.14
Fjoh_0707	FJOH_RS03710 porZ	T9SS type A sorting domain-containing protein PorZ	T	1.48
Fjoh_1448	xrtF	Exosortase family protein XrtF	S	3.82
Fjoh_1449	FJOH_RS07530	Exosortase F system-associated protein	No COG	3.74
Fjoh_1555	porV	T9SS outer membrane channel protein PorV	I	2.69
Fjoh_1556	porU	T9SS sortase PorU	S	1.33
Fjoh_1653	sprA	Cell surface protein SprA	S	3.25
Fjoh_2379	FJOH_RS12370	LysM peptidoglycan-binding domain-containing protein	M	2.5

^a List of genes that were validated by constructing in-frame deletions. The complete list of genes identified is provided in Table S1. The COG categories are abbreviated as follows: E, amino acid metabolism and transport; T, signal transduction; S, unknown function; I, lipid transport and metabolism; and M, cell wall/membrane/envelope biogenesis.