TABLE 3.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Description | Source(s) or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli-S17-1 λpir | Strain used for delivery of mariner transposon | ATCC |
| E. coli-S17-1 | Strain used for cloning/conjugation | ATCC |
| F. johnsoniae UW101 | Wild-type F. johnsoniae ATCC 17061 | Commonly found in soil and freshwater (51) |
| F. johnsoniae CJ1827 | rpsL2; (Smr) parent strain for construction of all deletion mutants | Reference 21 |
| P. koreensis CI12 | Wild-type P. koreensis | Reference 10 |
| B. cereus UW85 | Wild-type B. cereus | Reference 10 |
| FJ0334 | rpsL2 Δfjoh_0334 (Smr) | This study |
| FJ0347 | rpsL2 Δfjoh_0347 (Smr) | This study |
| FJ0651 | rpsL2 Δfjoh_0651 (Smr) | This study |
| FJ0707 ΔporZ |
rpsL2 Δfjoh_0707 (Smr) | This study |
| FJ1448 ΔxrtF |
rpsL2 Δfjoh_1448 (Smr) | This study |
| FJ1449 | rpsL2 Δfjoh_1449 (Smr) | This study |
| CJ2130 ΔporV |
rpsL2 ΔporV (Smr) | Reference 43 |
| CJ2116 ΔporU |
rpsL2 ΔporU (Smr) | Reference 43 |
| CJ2302 ΔsprA |
rpsL2 ΔsprA (Smr) | Reference 40 |
| FJ2379 | rpsL2 Δfjoh_2379 (Smr) | This study |
| Plasmids | ||
| pSAMFjoh_2 | Transposon mutagenesis vector for F. johnsoniae; Apr (Emr) | Reference 27 |
| pRR51 | rpsL-containing suicide vector; Apr (Emr) | Reference 21 |
| pCP23 | E. coli-F. johnsoniae shuttle plasmid; Apr (Tcr) | Reference 55 |
| pCP0334 | 1134 bp fragment spanning fjoh_0334 was amplified with primers Fjoh_0334_CP_UP and Fjoh_0334_CP_DOWN and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
| pCP0347 | 729 bp fragment spanning fjoh_0347 was amplified with primers Fjoh_0347_CP_UP and Fjoh_0347_CP_Down and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
| pCP0651 | 434 bp fragment spanning fjoh_0651 and its presumed promoter was amplified with primers Fjoh_0651_CP_UP and Fjoh_0651_CP_DOWN and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
| pCP0707 | 2283 bp fragment spanning fjoh_0707 was amplified with primers Fjoh_0707_CP_UP and Fjoh_0707_CP_DOWN and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
| pCP1448 | 873 bp fragment spanning fjoh_1448 and its presumed promoter was amplified with primers Fjoh_1448_CP_UP and Fjoh_1448_CP_DOWN and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
| pCP1449 | 447 bp fragment spanning fjoh_1449 was amplified with primers Fjoh_1449_CP_UP and Fjoh_1449_CP_DOWN and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
| pSSK03 | pCP29 carrying porV; Apr (Cfr Emr) | Reference 43 |
| pSSK04 | pCP23 carrying porU; Apr (Tcr) | Reference 43 |
| pSN48 | pCP23 carrying sprA; Apr (Tcr) | Reference 40 |
| pCP2379 | 2,416 bp fragment spanning fjoh_2379 and its presumed promoter was amplified with primers Fjoh_2379_CP_UP and Fjoh_2379_CP_DOWN and inserted into BamHI-SphI sites of pCP23; Apr (Tcr) | This study |
a
Antibiotic resistances are as follows: streptomycin, Smr; ampicillin, Apr; erythromycin, Emr; tetracycline, Tcr; cefoxitin, Cfr. Antibiotic resistances within parentheses are expressed in F. johnsoniae but not in E. coli, and those without parentheses are expressed in E. coli but not in F. johnsoniae.