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. 2024 Mar 11;12(3):e008554. doi: 10.1136/jitc-2023-008554

Figure 4.

Figure 4

Inhibitors of ROS and ferroptosis suppress mefloquine+IFN-γ-induced ferroptosis. (A) The ROS levels in A375 and A549 cells after Mef stimulation (4 µM, 6 hours) alone or in the presence of a ferroptosis inhibitor (ferrostatin-1, Fer-1; 1 µM, 6 hours) were determined (n=3 replicate cultures for each cell type). (B) The ROS levels in A375 and A549 cells after Mef stimulation (4 µM, 6 hours) alone or in the presence of an ROS inhibitor (N-acetylcysteine (NAC), 2.5 µM, 6 hours) were determined (n=3 replicate cultures for each cell type). (C) The lipid peroxidation levels in A375 and A549 cells after Mef stimulation (4 µM, 2 hours) alone or in the presence of Fer-1 (1 µM, 2 hours) were determined (n=3 replicate cultures for each cell type). (D) The numbers of A375 and A549 cells in cultures supplemented with Mef (4 µM), IFN-γ (20 ng/mL) or Fer-1 (1 µM) for 48 hours are shown (n=3 replicate cultures for each cell type). (E) The numbers of A375 and A549 cells in culture supplemented with Mef (4 µM), IFN-γ (20 ng/mL) or NAC (2.5 µM) for 48 hours are shown (n=3 replicate cultures for each cell type). NAC and Fer-1 will treat the cells 8 hours in advance before adding Mef. All the data are presented as the mean (±SD). One-way analysis of variance was performed for comparisons among multiple groups. Statistical significance was assessed according to statistical methods (*p<0.05; **p<0.01; and ***p<0.001). IFN, interferon; Mef, mefloquine; ROS, Reactive oxygen species.