FIGURE 5.

Profile counts for Reelin positive cells shows CORT reduced SGZ Reelin counts after 2.6 cycles, and sex differences to localization of Reelin count alterations after Reelin treatment. Results of Reelin positive cell counts for the SGZ of the DG from rats treated with 1.3 and 2.6 cycles of chronic stress alongside representative images of Reelin immunohistochemistry. (A): Reelin count estimate totals for animals exposed to 1.3 cycles of chronic stress showing no change to Reelin counts after 1.3 cycles of chronic stress. (B): Reelin positive cell count estimates of the dorsal blade of the DG showing no change after 1.3 cycles of chronic stress. (C): Reelin positive cell count estimates of the ventral blade of the DG showing no change after 1.3 cycles of chronic stress. (D): Reelin count estimate totals for animals exposed to 2.6 cycles of chronic stress showing reduced Reelin counts after 2.6 cycles of chronic stress in both males and females that were partially recovered with a single intravenous Reelin injection. (E): Reelin positive cell count estimates of the dorsal blade of the DG showing reduced counts after 2.6 cycles of chronic stress that were partially recovered in females but not males. (F): Reelin positive cell count estimates of the ventral blade of the DG showing no significant reduction to ventral blade counts after 2.6 cycles of chronic stress, however an intravenous injection of Reelin significantly increased Reelin positive cell count estimates for males and not females. (G): Example photomicrograph at 2.5X magnification showing Reelin positive cells in the DG from a rat exposed to vehicle/vehicle injections with demonstration of how dorsal and ventral blades of the DG were differentiated. (H): Photomicrograph at 2.5X magnification showing DG Reelin positive cells from a rat exposed to CORT/vehicle injections. (I): Photomicrograph showing Reelin positive cells in the DG from a rat exposed to CORT/Reelin injections.