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Replication following transfection of SIVMne chimeras in CEMx174 cells. CEMx174 cells were transfected with each chimeric virus clone as well as SIVMneCL8. At 3- to 4-day intervals for 4 weeks after transfection, virus replication and spread were monitored by assaying for SIVp27gag in the culture supernatants. To determine the levels of SIVp27gag antigen, dilutions of supernatant were tested in the linear range of the assay. SIVp27gag is plotted as a function of days posttransfection.
