FIG. 3.
Infection of primary macaque cells. M. nemestrina blood-derived cells were infected with virus expressed from CEMx174 cells transfected with the plasmid viral clones. Infection and virus replication were monitored by an assay for SIVp27gag. (A) PBMCs (8 × 105) were infected at an MOI of 0.01; (B) macrophage cultures (2.5 × 105 cells) were infected at an MOI of 0.001. The data represent an average of duplicate infection experiments. In panel B, 170 refers to a full-length molecular clone, SIVMne170, described previously (24).