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. 2023 Sep 21;20(3):541–556. doi: 10.1080/15548627.2023.2261758

Table 1.

Summary of antibodies used in western blot, immunochemistry, immunofluorescence, co-immunoprecipitation, affinity isolation, and CETSA.

Antibody Concentration
Company
for WB for IHC for IF for Co-IP Cat. No.
AFAP1L2 / 1:200 / / Ab106433 Abcam
1:1000 / 1:200 / 17183–1-AP Proteintech
/ / / 1:50 12684 Cell Signaling Technology
p-SRC 1:1000 / / / AF3161 Affinity bioscience
/ / / 1:50 4211 Cell Signaling Technology
SRC 1:1000 / / 1:50 2109 Cell Signaling Technology
p-FUNDC1 1:1000 / / / TP30110 Huabio
FUNDC1 1:1000 / / / ab224722 Abcam
LC3B 1:1000 / / / 3868 Cell Signaling Technology
/ / 1:200 / Ab51520 Abcam
GAPDH 1:5000 / / / 60004–1-lg Proteintech
TOMM20 1:1000 / 1:200 / Ab186735 Abcam
LAMP1 / / 1:200 / 55273–1-AP Proteintech
ABCC1 1:1000       ab180960 Abcam
ABCB1 1:1000       ab170904 Abcam
Normal rat IgG / / / 1:50 3900S Cell Signaling Technology
HRP-Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) 1:10000 / / / BA1054 Boster
HRP-Conjugated AffiniPure Goat Anti-mouse IgG (H+L) 1:10000 / / / BA1050 Boster
AMCA – conjugated Affinipure Goat Anti-Rabbit IgG(H+L) / / 1:500 / SA00010–2 Proteintech
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) / / 1:500 / 5257 Cell Signaling Technology
Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) / / 1:500 / ab150077 Abcam