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. 2024 Jan 2;22(23-24):2552–2565. doi: 10.1080/15384101.2023.2298027

Figure 1.

Figure 1.

EZH2 and HMGA1 expression levels are upregulated in ATC-derived cell lines and tissues. (a) HMGA1 and EZH2 mRNA expression levels in a panel of ATC-derived cell lines (ACT1, FRO, 8505c and SW1736). The expression levels of normal human thyroid-derived primary cells were set equal to 1. Data are shown as mean ± SD (n = 3) (b) HMGA1 and EZH2 protein expression in the same ATC-derived cell lines and in normal human thyroid-derived primary cells. 50 μg of total cellular lysate was loaded per lane in reducing conditions. GAPDH was used as protein loading control. NT = normal thyroid tissue. (c) Twelve ATC tissues were analyzed for HMGA1 and EZH2 expression with respect to three normal thyroid tissues. The mean of normal samples was set equal to 1. Data are shown as mean ± SD (n = 3).