TABLE 1.
Strains used in this study.
| Strain | Description | References |
|---|---|---|
| Escherichia coli JM109 | For routine plasmid maintenance and cloning | Yanisch-Perron et al. (1985) |
| Escherichia coli ET12567/pUZ8002 | Methylation-deficient strain for isolation of non-methylated DNA and conjugating plasmids into Streptomyces | MacNeil et al. (1992) |
| Escherichia coli TOP10 | For protein expression | Invitrogen |
| Streptomyces peucetius var. caesius ATCC 27952 | Derived from Streptomyces peucetius ATCC 29050, producer of daunorubicin and doxorubicin | Arcamone et al. (1969) |
| Streptomyces peucetius G001 | Derived from ATCC 27952 by NTG mutagenesis; increased production of daunorubicin | Lambert and Ylihonko (2008) |
| MAG301 | G001 + pRDS | This work |
| MAG302 | G001 ΔdnrS | This work |
| MAG303 | G001 ΔdnrS + pRDS | This work |
| MAG304 | G001 ΔdnrS + pRDS + pGWS1432 | This work |
| MAG305 | G001 ΔdnrS + pGWS1433 | This work |
| MAG306 | G001 ΔdnrS + pRDS + pGWS1432 + pGWS1434 | This work |
| MAG307 | G001 ΔdnrS + pRDS + pGWS1432 + pGWS1435 | This work |
| MAG308 | G001 ΔdnrS + pRDS + pGWS1432 + pGWS1436 | This work |
| MAG309 | G001 ΔdnrS + pGWS1433 + pGWS1437 | This work |