Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 1 mL TB Syringe | BD | 309649 | |
| 1.5 mL Eppendorf Tubes | USA Scientific | 1415-2500 | |
| 14 mL, Open-Top Thinwall Ultra-Clear Tube | Beckman Coulter | 344060 | Compatible with SW 40 Ti |
| 23 Gauge Precision Glide Hypodermic Needle | BD | 305145 | |
| 26.3 mL, Polycarbonate Bottle with Cap Assembly | Beckman Coulter | 355618 | Compatible with Ti70 |
| 3.5 mL, Open-Top Thickwall Polypropylene Tube | Beckman Coulter | 349623 | Compatible with TLA-100.3 |
| 50 mL Falcon Tubes | Fisher Scientific | 14-432-22 | |
| Amicon Ultra-15 Centrifugal Filter Unit | Millipore Sigma | UFC901024 | |
| Aprotinin | Sigma-Aldrich | A6279 | 1 mg/mL in diH2O |
| Avanti J-26 XP Centrifuge | Beckman Coulter | B22984 | <26,000 rpm |
| Benchtop HDPE Dewar Flask | Thermo Scientific | 5028U19 | |
| C57BL/6J Mice | The Jackson Labs | 000664 | |
| Centrifuge 5810R | Eppendorf | EP022628168 | <14,000 rpm |
| complete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets | Roche | 11873580001 | Add 1 tablet per 50 mL of solution |
| Curved Forceps | Fine Science Tools | 11273-20 | |
| Fine Surgical Scissors | Fine Science Tools | 8r | |
| Glas-Col Tissue Homogenizing System | Cole-Parmer | UX-04369-15 | |
| Graefe Forceps | Fine Science Tools | 11650-10 | |
| High-Speed Polycarbonate Round Bottom Centrifuge Tubes | ThermoFisher | 3117-0500 | Compatible with JA20 |
| Isofluorane | Henry Schein Animal Health | NDC 11695-6776-2 | |
| JA-20 Rotor | Beckman Coulter | 334831 | |
| Leupeptin | American Bio | AB01108 | 1 mg/mL in diH2O |
| N-[2-Hydroxyethyl] piperazine-N’-[2-ethanesulfonic acid] (HEPES) | American Bio | AB00892 | |
| Optima L-80 XP Ultracentrifuge | Beckman Coulter | <100,000 rpm | |
| Optima TLX Ultracentrifuge | Beckman Coulter | <120,000 rpm | |
| Pepstatin A | Thermo Scientific | 78436 | 1 mg/mL in DMSO |
| Phenylmethylsulfonyl fluoride (PMSF) | American Bio | AB01620 | |
| Pierce BCA Protein Assay Kit | Thermo Scientific | 23335 | For determination of protein concentration |
| Pipette Tips | |||
| Serological Pipettes | |||
| Sucrose | Sigma-Aldrich | S0389 | |
| Surgical Scissors | Fine Science Tools | 14002-12 | |
| SW 40 Ti Swinging-Bucket Rotor | Beckman Coulter | 331301 | |
| Teflon-Coated Pestle and Mortar Tissue Grinder | Thomas Scientific | 3431D94 | |
| Ti70 Rotor | Beckman Coulter | 337922 | |
| TLA-100.3 Rotor | Beckman Coulter | 349490 | |
| Tube Revolver | Dot Scientific | DTR-02VS |