FIG. 6.

Characterization of MA and MtplA RNA species by RT-PCR. Cytoplasmic poly(A)-containing (lanes 2, 4, 6, 8, 10, and 12) or poly(A)-lacking (lanes 3, 5, 7, 9, 11, and 13) RNAs isolated from Ad5/MtplA-, Ad5/MA-, or Ad5-infected cells following exposure to dexamethasone for 1 h at 11 h after infection were analyzed by RT-PCR with a 3′ primer complementary to sequences near the β-actin poly(A) addition site and a 5′ primer corresponding to positions +21 to +44 of mouse mammary tumor virus (MMTV) transcription unit A or to the 5′ end of the tripartite leader sequence (Tpl). The lengths (in kilobase pairs) of DNA markers (lane 1) are indicated on the left.