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The IE86 protein is essential for the activation of the HCMV DNA polymerase (pol) gene promoter. U373MG cells were cotransfected with the pol-luciferase reporter and increasing amounts of the IE gene expression vectors RSVIE86, RSVIE72, and RSVIE55 and a β-galactosidase control vector. Luciferase activity was normalized to β-galactosidase activity. The data represent the results of three independent experiments.
