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IE86 protein enhances the DNA binding activity of Sp1. (A) U373MG (lane 2), U373-IE86 (lane 3), HeLa (lane 5), and HeLa-IE86 (lane 6) cell nuclear extracts were incubated with a radiolabeled Sp1 consensus oligonucleotide. (B) U373-IE86 nuclear extracts were incubated with the same Sp1 probe and a 50-fold excess of an unlabeled oligonucleotide (oligo) or polyclonal antibodies (Ab) as indicated. The unlabeled arrows indicate the Sp1 DNA-binding complex. SS, supershifted complex.
