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The factor(s) present in HeLa cells inhibits IE86-mediated DNA-binding activity of Sp1. U373-IE86 nuclear extract (2.5 μg) was incubated with radiolabeled IR1 (A) or Sp1 (B) consensus oligonucleotides, respectively. Increasing amounts of HeLa (lanes 2 to 4) or HeLa-IE86 (lanes 5 to 7) nuclear extract were added as indicated. U373-IE86 nuclear extract plus probe was used as a control (Lanes 1). The arrows indicate Sp1 DNA-binding complex.
