FIG. 6.

Identification of an IR1-specific DNA-binding complex in U373MG cells expressing IE86. Five-microgram quantities of nuclear extract from U373MG and U373-IE86 (A) or HeLa and HeLa-IE86 (B) cell lines were incubated with ³²P-labeled IR1 oligonucleotide. IR1 binding was analyzed by EMSA. (C) U373MG-IE86 nuclear extract was incubated with labeled IR1 oligonucleotide and a 50-fold excess of either unlabeled wild-type (IRwt) or mutant (IRmut) IR1 oligonucleotides. The arrows indicate the specific complex. NS, nonspecific complexes.