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Neither CRS nor EAIE2 IE86 cis elements can compete with the IR1-DNA complex. Nuclear extracts isolated from the U373-IE86 stable cell line were incubated with a radiolabeled IR1 oligonucleotide and a 50-fold excess of unlabeled CRS or EAIE2 competitor oligonucleotides as indicated. The arrow indicates the IR1 complex. wt, wild type; mut, mutant.
