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. 2024 Mar 13;10(11):eadk6906. doi: 10.1126/sciadv.adk6906

Fig. 2. Macrophage mechanosensing is mediated by cytoskeletal remodeling.

Fig. 2.

(A) Quantification of migration distance and speed of BMDMs cultured in low- or high-collagen gels after stimulation with IL-4. (B and C) Representative confocal images (B) and quantification of the sphericity (C) of BMDMs cultured in low- or high-collagen gels and stimulated with IL-4. Cells were stained with phalloidin (magenta) to visualize F-actin and 4′,6-diamidino-2-phenylindole (DAPI) (blue) to visualize the nucleus. Scale bars, 10 μm. (D) Relative gene expression in IL-4–stimulated BMDMs cultured in low- or high-collagen gels with dimethyl sulfoxide (DMSO) or latrunculin A (Lat A). (E) Relative gene expression in IL-4–stimulated BMDMs cultured in low- or high-collagen gels with DMSO or nocodazole (Noco). Data are represented as means ± SD. Student’s t test [(A) and (C)] and two-way analysis of variance (ANOVA) [(D) and (E)] are used for statistical analysis. **P < 0.01, ***P < 0.001, and ****P < 0.0001.