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. 2024 Feb 8;19(3):366–382. doi: 10.1016/j.stemcr.2024.01.002

Figure 4.

Figure 4

Motor neurons preferentially recruit LGALS8 to ubiquitylated lysosomes

(A) Representative images of galectin immunofluorescence in NPCs following 1 h of 500-μM LLOME treatment. N = 2 independent experiments. Scale bar, 10 μm.

(B) Representative images of galectin immunofluorescence in motor neurons following 1 h of 500-μM LLOME (left). Airyscan images of motor neurons following LLOME treatment co-stained with LAMP1 and LGALS8 show colocalization (right). N = 2 independent experiments. Scale bars, 10 μm (left) and 1 μm (right).

(C) Percent cells with LGALS8 puncta after 1 h of LLOME at indicated doses. N = 3 independent experiments.

(D and E) Representative images of LLOME-treated motor neurons co-stained for LGALS8 and ubiquitin (D) or p62 (E) demonstrating colocalization. N = 3 independent experiments. Scale bar, 10 μm.

(F) Representative images of TFEB staining. Quantification of nuclear TFEB fluorescence intensity. Scale bar, 10 μm. N = 3 independent experiments. All data expressed as means ± SEM. ns = nonsignificant. One-way ANOVA with Dunnett’s multiple comparison test (C, F). See also Figure S6.