Skip to main content
. 2024 Feb 8;19(3):366–382. doi: 10.1016/j.stemcr.2024.01.002

Figure 7.

Figure 7

p97 inhibition rescues persistence of damaged lysosomes

(A) Schematic of experimental design.

(B) Representative images LGALS8 puncta in 392.1 motor neurons in untreated, LLOME treated, or after release. All conditions contain CB-5083. Scale bar, 10 μm.

(C) Quantification of LGALS8 puncta in 392.1 and KOLF2.1 motor neurons 24 h after LLOME treatment with and without CB-5083 co-treatment. N = 3 independent experiments.

(D) Representative images of LysoSensor DND-189 live-cell imaging in untreated and CB-5083-treated 392.1 motor neurons.

(E) Quantification of DND-189 fluorescence in untreated (solid bars) and CB-5083 treated (shaded bars) motor neurons. N = 4 independent experiments. Scale bar, 10 μm.

(F) Quantification of 392.1 motor neuron viability before, during, and after LLOME and CB-5083 co-treatment. N = 4 independent experiments. All data expressed as means ± SEM unless otherwise indicated. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Two-way ANOVA with Sidak’s (C, D) or Dunnett’s (F) multiple comparison test. See also Figure S7.