. 2024 Feb 13;300(3):105747. doi: 10.1016/j.jbc.2024.105747

Table 2.

Cloning primers used in this study

Gene	Domain/protein	Primers (5′-3′)
ugt74b1	N_B1	AATTCATATGGCGGAAACAACTCCCA (Fwd/NdeI)
	N_B1	AACTCGAGTCAACCATAGTCTTTATCATCTTCCATCC (Rev/XhoI)
	C_B1	ATACATATGGGTGCGAGTCTGTTGAAAC (Fwd/NdeI)
	C_B1	ATAATCACGAGACCTTACTTCCCTAAACTCTCTATAAACTCGTTAATGCT (Rev/BsaI)
ugt74c1	Full-length	CGGAATTCAGTGAAGCAAAGAAGGGTCACG (Fwd/EcoRI)
	Full-length	CAAGCGGCCGCTTAAGTCAAAAGAGCAACAAACTCA (Rev/NotI)
	N_C1	AATTCATATGAGTGAAGCAAAGAAGGGT (Fwd/NdeI)
	N_C1	AACTGGATCCTGAGTAATCTTTGTCTTCTGGCAA (Rev/BamHI)
	C_C1	AATTCATATGGAACTCGAGAACTCCAAGA (Fwd/NdeI)
	C_C1	ATAATCACGAGACCTGACTTAAGTCAAAAGAGCAACAAACTCAT (Rev/BsaI)
ugt74b1	N_B1-C_C1	AATTCATATGGCGGAAACAACTCCCA (Fwd/NdeI)
ugt74b1	N_B1-C_C1	AATGGTACCATAGTCTTTATCATCTTCCATCCGATC (Rev/KpnI)
ugt74c1	N_B1-C_C1	AATGGTACCGAGAACTCCAAGACAGAGCCAGAC (Fwd/KpnI)
ugt74c1	N_B1-C_C1	TTATGCTAGTTATTGCTCAGCGGTGG (Rev)

Underlined sequences correspond to added restriction enzyme cleavage sites. Fwd: upstream; Rev: downstream.