Glucose metabolism in HFD fed mice. (A) Blood glucose level and (B) insulin plasma level during ipGTT and consequential (C) calculated HOMA-IR in 12-week-old mice. (D) Glucose infusion rate (GIR), (E) Whole body glucose uptake during steady state, (F) endogenous glucose production rate (EGP) and (G) suppression of EGP during hyperinsulinemic-euglycemic clamp in 26-week-old mice. (H) 2-DG uptake in skeletal muscles (QD, EDL, GAS), heart, WAT and BAT. (I) Free fatty acids during hyperinsulinemic-euglycemic clamp. Data represents mean ± SEM (n = 4–8). ∗P < 0.05, ∗∗P < 0.01.