FIG. 4.
Interference with repair by HBx and mutant derivatives. HepG2 cells were assayed by the HCR assay described in Materials and Methods with the test plasmid construct listed under each bar: Neor, neomycin-resistance; wt, wild-type HBx subtype adw2; amino acid numbers, residues mutagenized (point mutants with the changed codon indicated by the standard single-letter abbreviation) or retained (deletion constructs); X-rev, X gene in the reverse orientation; β-gal, β-galactosidase reporter. Repair for the pSV2neo control (black bar) was set to 100%, and repair for cells transfected with the other constructs was compared to that value (numbers above each bar). Values shown are the means of at least three independent experiments, and the error bars represent the standard deviations.