TABLE 1.
Correlation of HBx binding to UVDDB and HBx-induced inhibition of DNA repair
| HBV X proteina | Binding to UVDDBb | Inhibition of DNA repairc |
|---|---|---|
| HBx1–154 | + | + |
| Point mutations | ||
| HBx7 | + | + |
| HBx61 | − | + |
| HBx69 | − | − |
| HBx90–91 | − | − |
| Deletion mutations | ||
| HBx1–67 | − | − |
| HBx1–80 | − | + |
| HBx1–101 | + | + |
| HBx55–101 | + | + |
a
HBx proteins tested in the assays. Full-length HBx (aa 1 to 154) and HBx proteins containing point and deletion mutations were created as described in Materials and Methods.
b
Binding of HBx to UVDDB measured by yeast two-hybrid assay as described previously (37). +, binding; −, no binding.
c
Inhibition of DNA repair was measured in the host cell reactivation assay (see Materials and Methods). +, inhibition; −, no inhibition.