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Depletion of CBX2 inhibits CRC cells proliferation and arrests cell cycle. (A) WB is used to assess the KO efficiency in HCT116 and HT29 cells. (B, C) MTT (B) and colony formation assay (C) are performed to assess cell proliferation. (D) Flow cytometry analysis is used to detect cell cycle distribution. (E) WB is used to assess the alteration of cell cycle-related proteins (CDK4, CDK6 and CCND1). Data are shown as mean ± SD; ***P < 0.001.
