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. 2024 Feb 24;15(8):2123–2136. doi: 10.7150/jca.92633

Figure 5.

Figure 5

CBX2 regulates MAPK signalling pathway by stabilizing the expression of Mettl3. (A) Mettl3 expression level in HCT116 and HT29 cells with or without CBX2 KO. (B) WB of indicated proteins in 293A cells treated with or without MG132 (6 hours, 20μmol/L) after being transfected plasmid Mettl3, CBX2 or pc-DNA3.1. (C) WB of indicated proteins in HCT116 and HT29 cells with or without CBX2 KO treated with CHX (20 μg/ml). (D) WB of indicated proteins in 293A cells after being transfected plasmid Mettl3 and CBX2. (E) The IP assay is performed to detect whether CBX2 inhabits the ubiquitination of Mettl3 in 293A cells. (F) The m6A level of HCT116 cells is detected by EpiQuik m6A RNA Methylation Quantification Kit. (G) MTT assay is utilized to evaluate the proliferation of the indicated cells. (H) WB of indicated proteins in HCT116 CBX2 KO cells after being transfected plasmid CBX2 and pc-DNA3.1. Data are shown as mean ± SD; ***P < 0.001.