Figure 1.

Human γδ T cells migrate to mouse bone marrow after radiation, and their phenotype is identical to that in the in vitro-expanded cells and circulation. Mice were conditioned with (A) 1.5-Gy radiation, 25 mg/kg busulfan, or 300 µL IFA (1:1 with PBS) or (B) 1.5 Gy or 6-Gy radiation; then, γδ T cells were administered, and 24 hours later, the percentage of γδ T cells was assessed by flow cytometry (gated on CD3⁺ γδTCR⁺ cells). (A, B) Statistics analyzed by non-parametric one-way ANOVA with post hoc (p< 0.05 = *); the sample mean is denoted with a black line; n = 3–4 mice per condition. (C) Mice were conditioned with 6-Gy radiation and 24 hours later injected with γδ T cells, and phenotype markers of live γδ T cells were assessed by flow cytometry. Each combination of samples was statistically analyzed by Student’s t-test (p> 0.05 = ns; p< 0.05 = *; p< 0.01 = **). ns, not significant. The in vitro combinations were all non-significant except for CD27. The sample mean is denoted with a black line. In vitro data represent two biological replicates; in vivo studies represent n = 3 mice for each condition. IFA, incomplete Freund’s adjuvant; PBS, phosphate-buffered saline.