Extended Data Fig. 9. The T cell compartment educated in B cell-deficient mice facilitates the generation of autoantibodies.

(a) Mature CD4⁺ cells from either wild-type or B cell-deficient Mb1-Cre^KI/KI mice were transferred intravenously (i.v.) into Tcra^–/– recipient mice (d0) followed by subcutaneous immunization with PBS and CFA on the day after transfer (d1). Sera were collected on d32 after immunization and tested for autoantibodies (IgG) on cryosections of different organs dissected from Rag1^–/– mice using a secondary anti-mouse IgG (H + L) antibody. (b) Representative immunofluorescence stainings from various anatomical niches (choroid plexus, CNS vessel, kidney, skin) of n = 3 independent wild-type-educated sera and n = 4 independent Mb1-Cre^KI/KI-educated sera. Scale bar = 20 µm (except kidney: 50 μm). The diagram in (a) was created using Servier Medical Art and BioRender under a Creative Commons licence CC BY 3.0.