Extended Data Fig. 9. ID11b is potentially associated with tobacco smoking.

a. Spectra of signatures known to be associated with tobacco smoking (ID3 and SBS4) and ID11b. SBS92 (not shown) is a recently discovered signature also associated with tobacco smoking¹⁷. b. ID11b exposure is correlated with both SBS4 + SBS92 and ID3 exposures. PCAWG samples related to tobacco smoking, as indicated by nonzero exposures of SBS4 + SBS92, are selected. The per-sample exposures are then plotted for ID11b vs. SBS4 + SBS92, and ID11b vs. ID3. Pearson correlation coefficients and the corresponding p-values are annotated. c. ID (left) and SBS (right) signatures observed in cells exposed to different chemicals present in tobacco smoke. Mutation data is obtained from⁷. Background ID and SBS signatures averaged from control clones are removed. d. ID11b is required to explain the observed abundance of T insertions in PCAWG samples related to tobacco smoking. n₁ is defined as the number of C deletions at CC or CCC, characteristic of ID3, and n₂ is defined as the number of T insertions following a T, characteristic of ID11b (left). The ratio $\frac{n_2}{n_1+n_2}$ is then plotted for reconstructed spectra with MuSiCal assignment vs. observed spectra (middle), as well as for reconstructed spectra with PCAWG assignment vs. observed spectra (right). Without ID11b, the PCAWG assignment fails to explain the observed T insertions following a T.