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. 2024 Feb 21;627(8003):437–444. doi: 10.1038/s41586-024-07093-w

Fig. 2. Summary of key interacting modules of the 60S–UREL–UFC1–UFM1 complex.

Fig. 2

UFL1 interacts with the PTC (i). Top, the UFL1 C-terminal domain sits within the tRNA-binding groove and is clamped between 28S rRNA helices 69 (h96) and 38a (h38a). Bottom, the UFL1 loop (residues 436–448; solid line) reaches into the PTC and binds proximal to the P-site. Disordered connecting residues are shown as dashed lines. The substrate-recognition module (ii). UFL1 and CDK5RAP3 bind to RPL10a from the L1 stalk. The ribosome docking module (iii). The UFBP1 helix docks onto the ribosome adjacent to the peptide exit tunnel, near 28S rRNA helices 47 and 24, as well as RPL31. The catalytic module of the UREL complex (iv). UFM1 is bound by CDK5RAP3 and UFBP1, with UFM1 positioned directly above the C terminus of RPL26 near the site of UFMylation.