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. 2024 Feb 21;627(8003):437–444. doi: 10.1038/s41586-024-07093-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Main interactions between UREL and the 60S ribosome. Throughout, side chains are displayed as ball and stick and hydrogen bonds shown as black dashed lines. a, UFL1 N-terminus and UFBP1 C-terminus form a composite winged helix domain (pWH/pWH’). b, 60S ribosomal proteins RPL10a, RPL11, RPL36a, RPL36 and RPL13 interact with UFL1. c, Hydrogen bond network between UFL1 winged helix domains WH1 and WH2 and RPL13. d, UFL1 and CDK5RAP3 bind to RPL10a of the L1 stalk. Atomic model cartoon is coloured by protein and cryo-EM density shown in transparent grey. RBD is CDK5RAP3 ribosome binding domain. e, Hydrophobic residues at the RPL10a:UREL interface. f, Hydrogen bonding residues at the RPL10a:UREL interface. g, Overview of CDK5RAP3 domains. RBD is RPL10a binding domain. CCD is coiled-coil domain. UUBD is UFM1/UFBP1 binding domain. h, Main electrostatic interactions between CDK5RAP3 RBD and UFL1. I, Main electrostatic interactions between CDK5RAP3 CCD and UFBP1. j, Immunoblotting of membrane fractions from HEK293 WT, CDK5RAP3 KO or UFSP2, ODR4 double KO cells untreated or treated with 200 nM anisomycin for 60 min. Asterisk indicates empty lane. k, Superposition of CCDC47 (PDB ID 7tm3) with cryoEM structure of UREL:60S complex shown in cartoon representation reveals similar mode of ribosome docking.

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