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. 2024 Feb 21;627(8003):437–444. doi: 10.1038/s41586-024-07093-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Comparison between P-site regions of the 60S only map versus the UREL-bound 60S map, viewed at similar thresholds. Additional density was observed in the ligase-bound 60S map which corresponds to UFL1 (green). b, UFL1 loop positioning within PTC with surrounding rRNA bases shown. Cryo-EM density for UFL1 loop shown in transparent green. Arrows indicate loop N- and C-termini. c, 28S A4548 moves towards P-site to stack with UFL1 Y443. Transparent grey 60S model represents non-ligase bound 60S (PDB ID 6r7q). Opaque grey 60S model is UREL bound 60S. d, 28S U4452 moves towards A-site to sit proximal to G437. Transparent grey 60S model represents non-ligase bound 60S (PDB ID 6r7q). Opaque grey 60S model is UREL bound 60S. e, UFL1 N439 stacks with 28S rRNA A3908 and hydrogen bonds with G3807 (dashed line). f, UFL1 R441 stacks with 28S rRNA A4385 and hydrogen bonds with surrounding phosphates of 28S rRNA (dashed lines).