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. 2024 Feb 29;18:1336439. doi: 10.3389/fncel.2024.1336439

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Copyright © 2024 Perdaens, Bottemanne and van Pesch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Endogenous microRNAs in proliferating and differentiating CG-4 cells. The plots show (A) the relative expression in RT-qPCR (mean with SD of 2^−ΔΔCt) of each endogenous microRNA in CG-4 cells cultured in the proliferation or differentiation medium, as well as (B) the ΔCt of each microRNA with miR-103a-3p as reference (ΔCt = Ct(target microRNA)_sample – Ct(reference miR-103a-3p)_sample), which is representative of the relative abundance of each microRNA. Each color in (B) corresponds to a microRNA species, as indicated in the plot legend. The p-value (p) was calculated by a Mann–Whitney U-test, ns for not significant. Data were from four experiments with each condition in triplicate. PROL Co, proliferation control; DIFF Co, differentiation control; SD, standard deviation.