Figure 9.

NOD.Ifih1^R promotes depletion of β-cells and enhances B cell infiltration in pancreatic islets in 14-week-old males. (A) Frequency of B cells (CD45⁺B220⁺) in the pancreas of 14-week-old male mice (n=6 NOD.Ifih1^NR/NR , n=5 NOD.Ifih1^R/R ) by flow cytometry. (B) Representative images showing β-cells (insulin positive, green, top panel), B cells (white, middle panel), and merged images with DAPI (bottom panel). Scale bars: 50μM. (C-E) Graphs show (C) the percentage of β-cell-covered area/islet, (D) mean insulin intensity/islet, and (E) the percentage of B cell covered area/islet in pancreases of 14-week-old male mice (n=6 NOD.Ifih1^NR/NR , n=5 NOD.Ifih1^R/R ). (F-H) Graphs show (F) the percentage of β-cell-covered area/islet, (G) mean insulin intensity/islet, and (H) the percentage of B cell covered area/islet in pancreases of 14-week-old female mice (n=6 NOD.Ifih1^NR/NR , n=7 NOD.Ifih1^R/R ). P values were determined using student’s unpaired t tests. All data are mean ± SEM.