FIG. 2.
Representative ADCC assay results obtained with a panel of human anti-Env MAbs. CEM.NKR cells were chronically infected with the HIV-1 strains indicated. The left side of each panel shows results with the following controls: pooled seropositive serum at 10−3 dilution, irrelevant human (Hu) IgG1 (myeloma protein; ICN Immunobiological, Costa Mesa, Calif.) at 20 μg/ml, and rabbit antiserum against β2 microglobulin (Accurate Chemical, Westbury, N.Y.), a positive control against both uninfected and infected cells, at 33 μg/ml. Results with the latter control against uninfected cells were comparable to those seen against the four infected cell lines shown, while no specific lysis above background was seen against uninfected cells for any of the other Abs and MAbs in this experiment (data not shown). The right side of each panel shows results with various concentrations of each human MAb. The error bars represent standard deviations of duplicate points.