FIG. 5.

Results of ADCC assays using as targets A2.01 cells that were uninfected (A), infected with wild-type vaccinia virus (WR) (B), infected with vPE16, a vaccinia virus recombinant expressing Env of laboratory-adapted strain BH8 (C), or infected with vCB53, a vaccinia virus recombinant expressing a clade E primary isolate Env (D). The left side of each panel shows results with the following controls: irrelevant human (Hu) IgG1 (myeloma protein; ICN Immunobiological) at 40 μg/ml; rabbit antiserum against β2 microglobulin (Accurate Chemical), a positive control against both uninfected and infected cells, at 30 μg/ml; serum from a healthy individual recently immunized against vaccinia virus (anti-vaccinia serum) at 1/200; and HIVIG at 40 μg/ml. The right side of each panel shows results for two or more concentrations of each human or chimpanzee MAb tested. The error bars represent standard deviations of triplicate points.