FIG. 2.
Southern blot analysis of recombinant viruses containing mutations in the B5R ORF. (A) Schematic diagram of B5R region of vaccinia virus genomic DNA. The positions of the SnaBI sites and the expected sizes of the corresponding fragments are indicated. The arrows represent the coding region of the B5R gene. (B) Southern blot of DNA digested with SnaBI from mock-infected cells (Mock), cells infected with the wild-type strain of vaccinia virus (WR), the B5R deletion virus (W-B5R−), the recombinant virus with all four SCRs deleted (W-B5RΔSCR1–4), and the B5R revertant virus (W-B5Rrescue). Blots were probed with a 905-bp fragment that overlaps a region of the B5R ORF present in all recombinants. DNA molecular sizes (in kilobase pairs) are indicated. Arrows indicate the positions of the SnaBI digestion products.