Fig. 6.

Alamar Blue assay of rabbit ASCs seeded on nanocomposites PLGA/aCaP and PLGA/aCaP/CuO (5 %) that were sterilized with ethanol (+EtOH) or not (-EtOH) in addition to a sterilization with gentamycin/amphotericin B before seeding cells. As a control, cells were cultivated in tissue culture plates referenced as cells in well plate (no scaffold = no nanocomposite) (A). Immunohistochemical labeling for collagen I, fibronectin and α-SMA of cell-seeded scaffolds at day 7. Scale bars: 100 μm. (B). For negative controls, see the Supporting Information SI Fig. 2. Key: d3 = day 3; d7 = day 7 and d14 = day 14, EtOH = ethanol. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)