Scheme 1.

Overview of nanocomposite production and experiments. Fabrication of five bi-layered electrospun random fiber meshes with a thin layer of pristine PLGA (dark green) and a thick layer of a nanocomposite with the different compositions (1–5); and characterization by scanning electron microscopy (SEM), assessment of copper release kinetics over time and bacterial adherence (A), CAM assay with histological assessment and quantitative real-time PCR (qPCR) for VEGF and Ang1 gene expression (B), and seeding of adipose-derived stem cells on nanocomposites to measure proliferation by Alamar blue assay and immunohistochemical labeling after fixation (C).