TABLE 3.

Peak isotype-specific ASC responses to rotavirus in systemic lymphoid tissues after inoculation and challenge of gnotobiotic pigs with Wa HRV

Wa rotavirus inoculum groupb and ASC isotype	Peak no. of ASC (mean ± SEM)/5 × 105 MNCa
	Spleen				PBL
	PID 21–25		PCD 4		PID 21–25		PCD 4
	In vivo	In vitro	In vivo	In vitro	In vivo	In vitro	In vivo	In vitro
Group 1
IgG	0.1 ± 0.1	16	68 ± 21	305	0.5 ± 0.4	44	179 ± 113	1,522
IgA	0 (NDc)	4 (4)	5 ± 2 (13.6)	13 (23)	1 ± 0.61 (0.5)	2 (22)	28 ± 6 (6.4)	270 (5.6)
IgM	7 ± 4	ND	4 ± 2	ND	0.3 ± 0.3	ND	3 ± 2	ND
Group 2
IgG	34 ± 18	2,017	152 ± 24	3,450	237 ± 133	2,730	441 ± 166	11,500
IgA	0.3 ± 0.3 (113)	4.5 (448)	2 ± 1 (76)	0 (ND)	2 ± 1 (119)	14 (195)	20 ± 6 (22)	85 (135)
IgM	6 ± 3	ND	6 ± 5	ND	2 ± 1	ND	1 ± 0.6	ND
Group 3
IgG	80	225	370	ND	1,450	3,550	310	ND
IgA	1 (80)	0 (ND)	20 (19)	ND (ND)	11 (131.8)	0 (ND)	44 (7)	ND (ND)
IgM	6	0	17	ND	5	0	0	ND

^aNumbers of Wa rotavirus-specific ASC were assessed by ELISPOT assay at PID 21 to 25 (PCD 0) and PCD 4 (PID 25) in the duodenum, ileum, MLN, spleen, and PBL. In most of the tissues tested, the highest virus-specific IgG and IgA numbers were detected at PCD 4. Values in parentheses are ratios of IgG to IgA ASC based on numbers of ASC per 5 × 10⁵ MNC. 

^bData groups 1 and 2 represent four to six pigs for in vivo ASC responses and one or two pigs for in vitro ASC responses. For the latter responses, MNC from the indicated tissues were restimulated in vitro with semipurified rotavirus antigen at the time points noted. Data for group 3 pigs represents one or two pigs for in vivo and in vitro ASC responses. No standard errors of the means were calculated for the in vivo or in vitro ASC responses of the group 3 pigs because of the unavailability of adequate numbers of MNC from only one or two pigs at the time points shown. 

^cND, not determined.