TABLE 2.
MPEV exhibits a mosaic expression pattern in ES cells
| ES cell line and clone | Cloning efficiency (%) (% LacZ+ colonies)a
|
Neor colonies/ total colonies | LacZ+ colonies/ total colonies
|
||
|---|---|---|---|---|---|
| Without G418 | With G418 | Without G418 | With G418 | ||
| CCE | 33.0 (0) | 0.0 (0) | NAb | NA | NA |
| CCE-MPEVlacZneoR | |||||
| 6 | 18.2 (5.2) | 6.5 (2.2) | 0.36 | 0.35 | 0.36 |
| 9 | 27.3 (2.4) | 5.1 (0.6) | 0.19 | 0.09 | 0.12 |
| 13 | 30.6 (8.5) | 14.6 (3.9) | 0.48 | 0.28 | 0.27 |
Cells were plated on inactivated feeder cells in either the absence or presence of G418. Results are averages from two independent experiments.
NA, not applicable.