Table 2.

Turnover of PmHMGR at pH 7 and pH 9 in various buffers testing the inhibitive effect of sodium chloride in comparison to the crystallization and enzymatic assay buffer

Buffer conditions (pH 7)	Turnover pH 7 (min−1)	Turnover pH 9 (min−1)
100 mM Tris, 100 mM potassium phosphate, 100 mM glycine (enzymatic assay buffer)	270.9 ± 20.9	1367.94 ± 108.85
1.2 Ammonium sulfate, 100 mM ADA, and 32% glycerol (crystallization buffer)	19.80 ± 3.31 (92.6%)	190.11 ± 27.42 (86.1%)
1.2 M sodium chloride, 100 mM ADA, and 32% glycerol	36.92 ± 9.55 (86.4%)	124.35 ± 14.29 (90.9%)

Percentage drop in turnover in the presence of ammonium sulfate and sodium chloride are also included with turnover values.