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. 2024 Feb 13;300(3):105746. doi: 10.1016/j.jbc.2024.105746

Figure 2.

Figure 2

Oxygen consumption kinetic profiles for Erp41 with Ero1–Pdi1 complex.A, representative oxygen consumption trace of K. phaffii WT Ero1–Pdi1 complex in combination with 10-fold exogenous Komagataella phaffii Erp41 (solid line); control reaction with monomeric Ero1C136A without supplied Pdi1 (dashed line); reaction catalyzed by Ero1–Pdi1 complex without monomeric Pdi1 or Erp41 (dotted line). B, differentiated oxygen consumption trace of 1 μM WT Ero1–Pdi1 complex and 1 μM Erp41 fitted to a single step activation model. Residuals are shown above the fitted trace. C, titration effect of supplied Erp41 on activation rate. D, titration effect of supplied Erp41 on kcat, data was fitted to the classical Michaelis–Menten model. Data is shown as mean ± SD (n = 3–5). PDI, protein disulfide isomerase.