Figure 4.
Examination of native and non-native 3S species of BPTI from refolding with Erp41 at 7 μM.A, elution profile at the native 3S BPTI position for BPTI folded by Erp41 for 120 min (blue) and immediately after mixing (red). For comparison, the 3S elution profile of BPTI refolded for 120 min by PDI (black) is shown. B, kinetic profile of appearance and disappearance of the non-native 3S∗ species and native 3S BPTI species from Erp41 refolding. Quantification is based on the sum of peak intensities as a function of time, in the form of average and standard error (n = 3). BPTI, bovine trypsin inhibitor; PDI, protein disulfide isomerase.
