Figure 7.
Stopped-flow kinetics of reduction of the a’ domain active site by GSH.A, mechanism of GSH-mediated reduction including kinetic partitioning. B, representative traces of the kinetics of reduction of the a’ domain (W162F) in the presence of 1 mM, 5 mM, and 10 mM GSH. Residuals are shown for one fit to a single exponential function at 1 mM GSH. C, nonlinear regression of the kobs values on [GSH]. Data is shown as mean ± SD (n = 4–7).
