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. 2022 Apr 1;8(13):eabm2592. doi: 10.1126/sciadv.abm2592

Fig. 3. Numerical and experimental results for enriching particles using an AFBH-based dual transducer device.

Fig. 3.

(A) Left: A schematic for illustrating the mechanism of an AFBH-based dual transducer device. Right: A photo of a fabricated device with a pair of piezoelectric transducers for generating flexural waves. (B) Left: A simulated flexural wave field (out-of-plane displacement amplitude |uz|) in the substrate at a frequency of 147 kHz showing that an antinode can be generated at the AFBH center. Right: The corresponding acoustic streaming field in the fluid domain showing that streaming-induced drag forces (red arrows) converge at the antinode. (C) Time-lapse microscopy images showing that 10-μm fluorescent polystyrene particles can be gradually enriched at the antinode in the AFBH. Scale bars, 1 mm. For this experiment, the excitation voltage, frequency, droplet volume, and initial particle concentration are 10 Vpp, 147 kHz, 20 μl, and 8.25 × 105 ml−1, respectively. (D) Quantitatively characterized mean fluorescence intensities for droplets with different initial particle concentrations. For all the test groups, the excitation voltage, frequency, droplet volume, and duration of acoustic waves are kept as 10 Vpp, 147 kHz, 20 μl, and 3 min, respectively. a.u., arbitrary units.