TABLE 3.
Inhibition of T-tropic viruses by SDF-1α
| Genetic subtype | Virus | Use ofa:
|
Wild typeb
|
Δ-32/Δ-32b
|
|||
|---|---|---|---|---|---|---|---|
| CCR5 | CXCR4 | ID90 | ID50 | ID90 | ID50 | ||
| A | 92UG029 | − | + | >4.8 | 3.5 | ND | ND |
| B | 2076 cI.3 | + | + | 3.2 | 0.3 | 1.7 | 0.2 |
| B | C 7/86 | + | + | >4.5 | 0.2 | 2.6 | 1.2 |
| B | DH123 | + | + | >5.0 | >5.0 | 4.7 | 2.4 |
| B | SF2 | + | + | >4.6 | 0.3 | 3.0 | <0.3 |
| B | HC4 | − | + | 4.3 | 1.3 | 3.9 | 1.3 |
| B | NL4-3 | − | + | 3.5 | 0.4 | 0.7 | 0.4 |
| C | ZAM20 | − | + | 0.2 | <0.2 | ND | ND |
| C | 94ZW106 | − | + | 2.7 | 1.5 | ND | ND |
| D | UG270 | − | + | 3.9 | <1.8 | ND | ND |
| D | 92UG046 | − | + | 4.9 | 3.2 | ND | ND |
| D | 92UG024 | − | + | 4.5 | 2.3 | ND | ND |
| E | 94TH304 | − | + | >5.0 | >3.8 | ND | ND |
a
The test isolates’ abilities (+) or inabilities (−) to use CXCR4 and CCR5 to enter transfected U87MG-CD4 cells are recorded.
b
The median ID50s and ID90s (in micrograms per milliliter) for the CXC chemokine SDF-1α against T-tropic isolates in cells from individuals homozygous for wild-type or Δ-32 CCR5 alleles are recorded. The data represent the means of two independent experiments. A value marked by “>” indicates that 50 or 90% inhibition was not achieved at the highest SDF-1α concentration tested. A value marked by “<” indicates that 50 or 90% inhibition was always achieved at the lowest SDF-1α concentration tested. ND, not done.